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G3 (Bethesda) ; 10(9): 3399-3402, 2020 09 02.
Article in English | MEDLINE | ID: covidwho-695997

ABSTRACT

The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.


Subject(s)
Betacoronavirus/genetics , Open Reading Frames/genetics , Betacoronavirus/isolation & purification , COVID-19 , Cloning, Molecular , Coronavirus Infections/pathology , Coronavirus Infections/virology , Escherichia coli/metabolism , Humans , Pandemics , Plasmids/genetics , Plasmids/metabolism , Pneumonia, Viral/pathology , Pneumonia, Viral/virology , Potyvirus/genetics , SARS-CoV-2
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